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. 2020 Dec 23;4(2):e202000808. doi: 10.26508/lsa.202000808

Figure S2. Related to Fig 2. BAF complex and polycomb domains do not overlap genome-wide in synovial sarcoma.

Figure S2.

(A) Pie chart showing common genomic locations of polycomb domains in synovial sarcoma 3D cultures. (B) Heat maps showing ChIP-seq signals for SMARCA2/4, H3K4me1, H3K4me3, H3K27ac, and H3K27me3 at polycomb domains common to synovial sarcoma patient-derived tumor organoids. Strong signals are detected for the polycomb repressive mark H3K27me3 but not for SMARCA2/4. 20-kb windows centered on H3K27me3 peaks are shown. (C) Pie chart showing genomic locations of narrow SMARCA2/4–binding sites common to synovial sarcoma patient-derived tumor organoids (bottom quartile identified in Fig 2A). (D) Heat maps showing ChIP-seq signals for SMARCA2/4, H3K4me1, H3K4me3, H3K27ac, and H3K27me3 at distal sites (top) and promoters (bottom) for narrow BAF complex–binding sites common to synovial sarcoma organoids. Marks of activity (H3K4me1, H3K4me3, and H3K27ac) are detected but not the polycomb repressive mark H3K27me3. 20-kb windows centered on SMARCA2/4–binding sites are shown.