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. 2020 Dec 11;4(2):e202000967. doi: 10.26508/lsa.202000967

Figure S10. Metaplasia marker expression is low in KRAS+ mice at the 2 wk time point.

Figure S10.

Corpus tissue from Hp−/KRAS+ and Hp+/KRAS+ mice obtained after 2 wk (N = 2 independent mouse experiments) was assessed for metaplasia via immunofluorescence microscopy. Images were taken with a Zeiss LSM 780 confocal microscope using 10× and 20× objectives and analyzed with Zen and Fiji software. Representative images are shown. Scale bars, 100 μm. (A) Stomachs were stained with antibodies against CD44v (green, no staining detected) and TFF3 (red), the lectin GS-II (blue), and DAPI (grey) in N = 3 staining experiments. Asterisks show examples of nonspecific (false-positive) TFF3 staining, which is not cell-associated, whereas specific staining is diffuse throughout the cytoplasm. (B) Stomachs were stained with antibodies against KI-67 (red) and pan-cytokeratin (blue), the lectin GS-II (green), and DAPI (grey) in N = 3 staining experiments.