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. 2020 Oct 12;216(4):1103–1116. doi: 10.1534/genetics.120.303623

Figure 2.

Figure 2

Impact of Δerg-10a and Δerg-10b on vegetative growth of N. crassa. (A) Growth of WT (FGSC 2489), Δerg-10a (FGSC 20057), Δerg-10b (N4-30), Δerg-10a/Δerg-10b (N4-38), Δerg-10a/Δerg-10b + erg-10a (MW_630), and Δerg-10a/Δerg-10b + erg-10b (MW_631) in MM tubes. Insets: in contrast to WT (i), Δerg-10a/Δerg-10b (ii) typically produces dark pigments along the glass wall of the tube. (B) Quantification of linear hyphal extension rates and sporulation. Values represent the mean ± SD from at least three independent experiments per strain (****P < 0.0001, ANOVA with Tukey’s post hoc test). (C) Ten-fold serial dilutions of conidia of the indicated strains were spotted onto sorbose-rich medium in the absence or presence of the polyene antifungal compound nystatin. FGSC, Fungal Genetics Stock Center; MM, minimal medium; WT, wild-type.