Table 1. Bacterial strains, phages, and plasmids used in this study.

Designation	Relevant characteristics	Source
Bacterial strains
DH5α	F−, Δ(argF-lac)169, ϕ80dlacZ58(M15), ΔphoA8, glnV44(AS), λ-,deoR481, rfbC1, gyrA96(NalR), recA1, endA1,thi-1,hsdR17	E. coli Genetic Stock Collection (CGSC) #12384
W3110	F−, λ-, IN(rrnD-rrnE)1, rph-1	CGSC #4474
BW25113 wt	F−, 567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514	CGSC #7636
DH5α (λpir)	DH5α lysogenized by phage λ434 (heteroimmune derivative) containing the pir gene	Gift from T. Charles. Kvitko et al. (2012)
W3110 (λ)	F−, λ+, λcI578, IN(rrnD-rrnE)1, rph-1	National BioResource Project (NBRP) #ME6104 (2009)
S17-1 (λpir)	TpR SmR recA, thi, pro, hsdR-M+RP4: 2-Tc:Mu: kmR,Tn7, λpir+	Gift from T. Charles. Matsumoto-Mashimo et al. (2004)
JW0427-1	ΔclpP::kmR, λ–	CGSC #8590 Baba et al. (2006)
JW0554-1	BW25113 isogenic derivative: ΔompT774::kmR	CGSC #8680 Baba et al. (2006)
JW0799-1	BW25113 isogenic derivative: ΔompX786::kmR	CGSC #11794 Baba et al. (2006)
JW0912-1	BW25113 isogenic derivative: ΔompF746::kmR	CGSC #8925 Baba et al. (2006)
JW0940-6	BW25113 isogenic derivative: ΔompA772::kmR	CGSC #8942 Baba et al. (2006)
JW1248-2	BW25113 isogenic derivative: ΔompW764::kmR	CGSC #9125 Baba et al. (2006)
JW1312-1	BW25113 isogenic derivative: ΔompG756::kmR	CGSC #11793 Baba et al. (2006)
JW1371-5	BW25113 isogenic derivative: ΔompN740::kmR	CGSC #9213 Baba et al. (2006)
JW2203-1	BW25113 isogenic derivative: ΔompC768::kmR	CGSC #9781 Baba et al. (2006)
JW3368-1	BW25113 isogenic derivative: ΔompR739::kmR	CGSC #10510 Baba et al. (2006)
JW3846-1	BW25113 isogenic derivative: ΔompL737::kmR	CGSC #10779 Baba et al. (2006)
JW5437-1	BW25113 isogenic derivative: ΔrpoS746::kmR	CGSC #11387 Baba et al. (2006)
JW0727-1	BW25113 isogenic derivative: ΔtolQ786:: kmR	CGSC #8793 Baba et al. (2006)
JW0728-1	BW25113 isogenic derivative: ΔtolR787:: kmR	CGSC #8794 Baba et al. (2006)
JW0729-3	BW25113 isogenic derivative: ΔtolA788:: kmR	CGSC #8795 Baba et al. (2006)
JW5100-1	BW25113 isogenic derivative: ΔtolB789:: kmR	CGSC #11174 Baba et al. (2006)
JW5437-1	BW25113 isogenic derivative: ΔrpoS746:: kmR	CGSC #11387 Baba et al. (2006)
JW5503-1	BW25113 isogenic derivative: ΔtolC732:: kmR	CGSC #11430 Baba et al. (2006)
JW0739-1	BW25113 isogenic derivative: ΔgalM728:: kmR	CGSC #8802 Baba et al. (2006)
JW0740-3	BW25113 isogenic derivative: ΔgalK729:: kmR	CGSC #8803 Baba et al. (2006)
JW0741-1	BW25113 isogenic derivative: ΔgalT730:: kmR	CGSC #8804 Baba et al. (2006)
JW0742-1	BW25113 isogenic derivative: ΔgalE740:: kmR	CGSC #8297 Baba et al. (2006)
JW1224-1	BW25113 isogenic derivative: ΔgalU745:: kmR	CGSC #9110 Baba et al. (2006)
JW2027-2	BW25113 isogenic derivative: ΔgalF731:: kmR	CGSC #9664 Baba et al. (2006)
JW2135-1	BW25113 isogenic derivative: ΔmglC775:: kmR	CGSC #9730 Baba et al. (2006)
JW2136-1	BW25113 isogenic derivative: ΔmglA776:: kmR	CGSC #9731 Baba et al. (2006)
JW2137-1	BW25113 isogenic derivative: ΔmglB777:: kmR	CGSC #9732 Baba et al. (2006)
JW2138-1	BW25113 isogenic derivative: ΔgalS778:: kmR	CGSC #9733 Baba et al. (2006)
JW2805-1	BW25113 isogenic derivative: ΔgalR762:: kmR	CGSC #10192 Baba et al. (2006)
JW2910-2	BW25113 isogenic derivative: ΔgalP789:: kmR	CGSC #10251 Baba et al. (2006)
JW3996-1	BW25113 isogenic derivative: ΔlamB732::kmR	CGSC #10877 Baba et al. (2006)
JW1667-5	BW25113 isogenic derivative: Δlpp-752::kmR	CGSC #9417 Baba et al. (2006)
JW0731-1	BW25113 isogenic derivative: Δpal-790::kmR	CGSC #8796 Baba et al. (2006)
Phages
T4D	Wild-type T4	Gift from G. Mosig (2009)
T4rIIΔ1586	Δ(rIIA-rIIB)	Gift from G. Mosig (2009)
λ (cI-857)	cI[ts]857, (rex+)	NBRP #ME6104 (2009)
λF7	λ, Dam15, imm21, cI[ts]857	(Gi Mikawa et al. 1996)
Plasmids
pUC19	High-copy number plasmid; MCS-lacZα, ApR	New England Biolabs (NEB) #N3041S, Whitby, Canada
pBSL199	ori R6K, lacIQ, Tn10, mob (RP4), IS10, ApR, and TcR	NBRP (2009), Alexeyev and Shokolenko (1995)
pHA1	(pUC19) [cI-rexA-rexB]; MCS- lacZα, ApR, pM-cI857-rexA-rexB-timm	This study
pHA2	(pBSL199) [cI-rexA-rexB]; IS10-TcR, pM-cI857-rexA-rexB-timm	This study