Table 6. Isolated integrant mutants attenuated for Rex activity.
| Strain | Wild-type level rex+ expressiona,b | Double rex+ expressionb,c |
|---|---|---|
| Controls | ||
| JW0427-1b | 1.0 | 1.0 |
| JW0427-1(λ)d | <1.0 × 10−7 | <1 × 10−7 |
| Isolates | ||
| JW-HA 1 | 2.3 × 10−5 | 4.0 × 10−6 |
| JW-HA 2 | 7.0 × 10−6 | 5.2 × 10−6 |
| JW-HA 3 | 1.5 × 10−5 | 1.7 × 10−5 |
| JW-HA 4 | 1.0 × 10−3 | 5.0 × 10−4 |
| JW-HA 5 | 1.7 × 10−5 | 5.0 × 10−6 |
| JW-HA 6 | 1.4 × 10−4 | 1.0 × 10−4 |
| JW-HA 9 | 5.0 × 10−6 | 4.0 × 10−6 |
| JW-HA 11 | 1.6 × 10−5 | 1.3 × 10−4 |
| JW-HA 12 | 1.3 × 10−5 | 1.6 × 10−4 |
| JW-HA 19 | 2.0 × 10−4 | 1.5 × 10−4 |
| JW-HA 20 | 8.0 × 10−6 | 6.8 × 10−6 |
| JW-HA 21 | 1.6 × 10−5 | 1.7 × 10−5 |
| JW-HA 25 | 1.8 × 10−5 | 4.0 × 10−6 |
a
Random insertion of the rexA-rexB transposable cassette into the chromosome renders insertional mutants genotypically rexA+rexB+ (rex+).
b
E. coli strain JW0427-1 (ΔclpP::kan) (rex−) used as negative control with relative T4rII EOP of 1.0. All values based on averaged EOP from three independent assays.
c
Transponants1 lysogenized with λ. Hosts carry two copies of rexA-rexB, one from transposon cassette and the second of λ lysogen.
d
E. coli strain JW0427-1(λ) (ΔclpP::kan) (rex+) used as positive control with relative T4rII EOP of 1.0.