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. 2020 Oct 19;216(4):1153–1185. doi: 10.1534/genetics.120.303748

Table 10.

The lack of spinal cord phenotypes in hmx2SU39 mutants is not due to genetic compensation

Embryo class WT hmx2SU39 mutants P value
Uninjected control 106.36 ± 1.75 109.67 ± 0.67 0.21^
WT-like 104.25 ± 5.30 98.33 ± 4.06 0.86^
Morphant-like 78.10 ± 2.50 71.25 ± 3.25 0.11+
All injected 85.57 ± 3.96 78.64 ± 4.56 0.26+

The spinal cord phenotypes of embryos (as assessed by slc17a6a/b expression) in the distinct phenotypic classes shown in Table 9 were analyzed on a compound microscope while blind to genotype. Values in columns 2 and 3 indicate the mean number of labelled cells in the spinal cord region adjacent to somites 6-10 ± SEM. Column 4 shows the P values from either a Wilcoxon-Mann-Whitney test (^, performed when data was not normally distributed) or from a type 2 Student’s t-test (+, performed when data was normally distributed and variances were equal) for the comparison of homozygous WT embryos to homozygous hmx2SU39 mutants for a particular classification (values on same row). All P values are rounded up to 2 decimal places. See Materials and Methods for more information on statistical tests.