Table 10.
The lack of spinal cord phenotypes in hmx2SU39 mutants is not due to genetic compensation
Embryo class | WT | hmx2SU39 mutants | P value |
---|---|---|---|
Uninjected control | 106.36 ± 1.75 | 109.67 ± 0.67 | 0.21^ |
WT-like | 104.25 ± 5.30 | 98.33 ± 4.06 | 0.86^ |
Morphant-like | 78.10 ± 2.50 | 71.25 ± 3.25 | 0.11+ |
All injected | 85.57 ± 3.96 | 78.64 ± 4.56 | 0.26+ |
The spinal cord phenotypes of embryos (as assessed by slc17a6a/b expression) in the distinct phenotypic classes shown in Table 9 were analyzed on a compound microscope while blind to genotype. Values in columns 2 and 3 indicate the mean number of labelled cells in the spinal cord region adjacent to somites 6-10 ± SEM. Column 4 shows the P values from either a Wilcoxon-Mann-Whitney test (^, performed when data was not normally distributed) or from a type 2 Student’s t-test (+, performed when data was normally distributed and variances were equal) for the comparison of homozygous WT embryos to homozygous hmx2SU39 mutants for a particular classification (values on same row). All P values are rounded up to 2 decimal places. See Materials and Methods for more information on statistical tests.