Table 11.

Genotypes of embryos from hmx3a^SU42/+ parents, injected with hmx3a morpholino, with different spinal cord phenotypes

Embryo class	WT	Het	hmx3aSU42 mutants	N value	P value
Uninjected control	22.5%	52.5%	25.0%	40	0.93
WT-like	31.25%	31.25%	37.5%	16	0.30
Morphant-like	20.5%	56.4%	23.1%	39	0.61

Spinal cord phenotypes were assessed by slc17a6a/b in situ hybridization. hmx3a translation-blocking morpholino-injected embryos from an incross of hmx3a^SU42/+ parents were visually inspected on a stereomicroscope and categorized as resembling either a “WT-like” (row 3) or “morphant-like” (row 4) spinal cord phenotype, compared to uninjected controls (row 2). Embryos were genotyped to identify homozygous WTs (column 2), heterozygotes (column 3) and homozygous mutants (column 4). Column 5 shows the total number of embryos in each phenotypic class. Column 6 shows the P value from Chi-squared tests performed to assess whether the observed ratios of genotypes in each phenotypic class was Mendelian. All P values are rounded up to 2 decimal places.