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. 2020 Oct 19;216(4):1153–1185. doi: 10.1534/genetics.120.303748

Table 14.

The incomplete penetrance of the spinal cord phenotype in hmx3aSU3 mutants is not due to genetic compensation

Embryo class WT hmx3aSU3 mutants P value
Uninjected control 106 ± 2.44 94.5 ± 2.62 0.01+
Weaker phenotype 102.75 ± 2.27 96.8 ± 1.77 0.05+
Morphant-like 70.22 ± 3.76 74.0 ± 2.75 0.72^
All injected 80.23 ± 4.01 81.86 ± 2.78 0.74+

The spinal cord phenotypes of embryos (as assessed by slc17a6a/b expression) in the distinct phenotypic classes shown in Table 13 were analyzed on a compound microscope while blind to genotype. Values in columns 2 and 3 indicate the mean number of labelled cells ± SEM. Column 4 shows the P values from either a Wilcoxon-Mann-Whitney test (^, performed when data was not normally distributed) or from a type 2 Student’s t-test (+, performed when data was normally distributed and variances were equal) for the comparison of homozygous WT embryos to homozygous hmx3aSU3 mutants for a particular classification (values on same row). All P values are rounded up to 2 decimal places. See Materials and Methods for more information on statistical tests. Statistically significant values are indicated in bold.