Table 14.
The incomplete penetrance of the spinal cord phenotype in hmx3aSU3 mutants is not due to genetic compensation
Embryo class | WT | hmx3aSU3 mutants | P value |
---|---|---|---|
Uninjected control | 106 ± 2.44 | 94.5 ± 2.62 | 0.01+ |
Weaker phenotype | 102.75 ± 2.27 | 96.8 ± 1.77 | 0.05+ |
Morphant-like | 70.22 ± 3.76 | 74.0 ± 2.75 | 0.72^ |
All injected | 80.23 ± 4.01 | 81.86 ± 2.78 | 0.74+ |
The spinal cord phenotypes of embryos (as assessed by slc17a6a/b expression) in the distinct phenotypic classes shown in Table 13 were analyzed on a compound microscope while blind to genotype. Values in columns 2 and 3 indicate the mean number of labelled cells ± SEM. Column 4 shows the P values from either a Wilcoxon-Mann-Whitney test (^, performed when data was not normally distributed) or from a type 2 Student’s t-test (+, performed when data was normally distributed and variances were equal) for the comparison of homozygous WT embryos to homozygous hmx3aSU3 mutants for a particular classification (values on same row). All P values are rounded up to 2 decimal places. See Materials and Methods for more information on statistical tests. Statistically significant values are indicated in bold.