Table 5.

Lateral line and otolith phenotypes of 40 embryos from hmx3a^SU42/+ parents injected with hmx2^MENTHU CRISPR reagents

Lateral line phenotype	Fused otoliths both ears	Normal otoliths both ears	Total
Strong	6	0	6
Medium	4	0	4
Weak	2	5	7
Normal	1	22	23
Total	13	27	40

Embryos from an incross of hmx3a^SU42/+ parents were injected with hmx2^MENTHU CRISPR reagents at the one-cell stage and assayed for lateral line primordium and otolith phenotypes at 30 hpf and 72 hpf, respectively. Rows 2–5 show stalled lateral line primordium migration phenotypes: normal = primordium in expected position (over somite 15 at 30 hpf); weak = primordium migration stalled by two or three somites; medium = primordium moderately stalled (over somite 6–7 at 30 hpf); strong = primordium not detected. Columns 2 and 3 show otolith phenotypes. Column 4 shows total number of embryos with each lateral line phenotype. Row 6 shows the total number of embryos with each otolith phenotype.