Figure 4.
BrdU incorporation in larval brains. Brains of third instar larvae expressing specified transgenes on orc6 deletion background were labeled with BrdU. Transgenes are as follows: (A) wild-type Drosophila Orc6, (B) Orc6-DH hybrid, (C) Orc6-HD hybrid, (D) wild-type human Orc6, (E) Drosophila Orc6-K23E mutant, and (F) Orc6-HD hybrid with the K23E mutation. Bars represents 50 µm. (G) Percentages of BrdU-labeled area relative to total brain area (n = 5). * indicates a statistically significant difference compared to Orc6-DmWT; ns indicates no significant difference.