Fig. 4.

The SARS-CoV-2 M protein interacts with RIG-I (a), MDA-5 (b), MAVS (c), and TBK1 (d) but not with IRF3 (e). f The truncated variants of SARS-CoV-2 M protein are depicted. g–i The interaction of RIG-I, MAVS, and TBK1 with the truncated variants of SARS-CoV-2 M protein. The HEK293T cells were transfected with the indicated plasmids for 24 h before coimmunoprecipitation with the anti-Flag magnetic beads. The pcDNA6B empty vector was used to balance the total amount of plasmid DNA in the transfection. The input and immunoprecipitates were immunoblotted with the indicated antibodies. Immunoblotting results are representative of two independent experiments. SARS-CoV-2 M protein, SCV2-M