FIGURE 5.

Effect of G . lucidum extracts on QGY7703 and SK-Hep1 cells apoptosis. (A) QGY7703 and SK-Hep1 cells were treated with GLE, G65 and G85 (150 μg/ml) for 24 h and analyzed for apoptotic and necrotic cell death using an Annexin-V: FITC Apoptosis Detection Kit. (B) QGY7703 and SK-Hep1 cells were treated with GLE, G65, G85 (150 μg/ml) for 48 h. The expression of Caspase 9, 3, and 7, PARP, cleaved Caspase 9 and cleaved PARP were determined by western blot. Data were shown as mean ± SD. *p < 0.05 (cleaved PARP),**p < 0.01 (apoptosis rate), ^## p < 0.01 (necrosis rate), vs control.