Figure 2.

elav-GAL4 Drosophila model of AD. A, TUNEL labeling in brains of 28-d-old flies. B, Kruskal–Wallis ANOVA analysis with Dunn’s multiple comparisons test showed that flies expressing C99^V717I(N = 6) and AB42 (N = 10) have a significant higher amount of TUNEL-labeled cell death compared with wt (N = 5; p = 0.0091, p = 0.0015, respectively). C, CIs were calculated by dividing the time a male spent in courtship to a total given time. Trainer and tester females: –, none; m, mated female. Box-and-whisker plots for CI show 10th, 25th, 75th, and 90th percentiles and mean (+). D, MIs were calculated as [100 [1 – (CI with training/mean of CI without training)]. Kruskal–Wallis ANOVA test followed by Dunn’s multiple comparisons test were used for statistical comparisons (N ≥ 20 for each genotype). elav-GAL4>UAS-C99^V717I and elav-GAL4>UAS-Aβ42 flies showed statistically significant lower MIs when compared with elav-GAL4>w¹¹¹⁸ but not elav-GAL4>UAS-C99^wt (p = 0.0423, p = 0.0001, and p = 0.1859, respectively) Data are shown as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001; n.s., not significant.