. 2020 Dec 23;7(6):ENEURO.0202-20.2020. doi: 10.1523/ENEURO.0202-20.2020

Table 2.

Statistical analysis

Behavior/experiment	Line	Data structure (normality)	Type of test	Power
Behavior/experiment	Line	Data structure (normality)	Type of test	Comparison	F/df	p
Validation of Drd2 CKO	1E	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 4.514, df = 10	p = 0.0011
Open field distance	2A	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,24) = 0.6405	p = 0.4314
				F2, time	F_(11,264) = 47.99	p < 0.0001
				(F1 × F2)	F_(11,264) = 0.8441	p = 0.5960
Open field % distance traveled	2B	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 1.781, df = 24	p = 0.0876
Rotarod	2C	No	Mann–Whitney, two-tailed	Control vs Drd2^Pet1-^CKO	M–W U = 142	p = 0.1899
Elevated plus maze (% time in open arm)	2D	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 1.250, df = 24	p = 0.2234
Tail suspension test	2E	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.3485, df = 24	p = 0.7305
Forced swim test	2F	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,24) = 0.2678	p = 0.6095
			F2, time	F_(5,120) = 8.916	p < 0.0001
			(F1 × F2)	F_(5,120) = 0.3090	p = 0.9067
Contextual fear conditioning (baseline freezing)	2G	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.6682, df = 24	p = 0.5104
Contextual fear conditioning (test freezing)	2G	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.0127, df = 24	p = 0.9900
Water T maze (%correct during acquisition)	2H	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,24) = 0.08249	p = 0.7764
				F2, time	F_(4,89) = 50.12	p < 0.0001
				(F1 × F2)	F_(4,89) = 0.6698	p = 0.6147
Water T maze (%correct during reversal)		Yes	Repeated-measures ANOVA	F1, genotype	F_(1,24) = 0.1631	p = 0.6899
				F2, time	F_(4,96) = 172.4	p < 0.0001
				(F1 × F2)	F_(4,96) = 1.477	p = 0.2153
ASR (M)	3C	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,25) = 0.0840	p = 0.7745
				F2, dB	F_(10,250) = 28.99	p < 0.0001
				(F1 × F2)	F_(10,250) = 0.3037	p = 0.9798
ASR habituation (M)	3D	Yes	Pearson r correlation	Control trial number × startle response	r = −0.195	p = 0.5893
ASR habituation (M)	3D	Yes	Pearson r correlation	Drd2^Pet1-CKO trial number × startle response	r = 0.136	p = 0.7079
ASR latency (M)	3E	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,25) = 2.425	p = 0.1319
				F2, dB	F_(10,250) = 21.67	p < 0.0001
				(F1 × F2)	F_(10,250) = 0.4722	p = 0.9071
ASR (F)	3F	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,29) = 13.26	p = 0.0011
				F2, dB	F_(10,29) = 35.29	p < 0.0001
				(F1 × F2)	F_(10,290) = 7.475	p < 0.0001
ASR habituation (F)	3G	Yes	Pearson r correlation	Control trial number × startle response	r = 0.1171	p = 0.7473
ASR habituation (F)	3G	Yes	Pearson r correlation	Drd2^Pet1-CKO trial number × startle response	r = 0.05165	p = 0.8873
ASR latency (F)	3H	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,29) = 0.3748	p = 0.5452
				F2, dB	F_(10,290) = 20.59	p < 0.0001
				(F1 × F2)	F_(10,290) = 1.058	p = 0.3953
PPI (M)	3I	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,12) = 0.6625	p = 0.4315
				F2, prepulse dB	F_(2,24) = 42.86	p < 0.0001
				(F1 × F2)	F_(2,24) = 4.104	p = 0.0293
PPI (F)	3J	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,10) = 0.6526	p = 0.4380
				F2, prepulse dB	F_(2,20) = 31.34	p < 0.0001
				(F1 × F2)	F_(2,20) = 1.609	p = 0.2249
ABR amplitude (M)	4C	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,15) = 1.770	p = 0.2032
				F2, peak	F_(2,30) = 59.09	p < 0.0001
				(F1 × F2)	F_(2,30) = 1.059	p = 0.3595
ABR latency (M)	4D	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,15) = 3.515	p = 0.0804
				F2, peak	F_(2,30) = 1171	p < 0.0001
				(F1 × F2)	F_(2,30) = 3.121	p = 0.0587
ABR threshold (M)	4E
5.6		Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.9535, df = 14	p = 0.3565
8		Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 1.894, df = 14	p = 0.0791
16		Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 1.103, df = 14	p = 0.2887
32		Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 2.129, df = 7	p = 0.0708
ABR amplitude (F)	4F	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,13) = 2.489	p = 0.1387
				F2, peak	F_(2,26) = 72.52	p < 0.0001
				(F1 × F2)	F_(2,26) = 0.0487	p = 0.9525
ABR latency (F)	4G	Yes	Repeated-measures ANOVA	F1, genotype	F_(1,13) = 0.0053	p = 0.9430
				F2, peak	F_(2,26) = 4360	p < 0.0001
				(F1 × F2)	F_(2,26) = 0.0822	p = 0.9213
ABR threshold (F)	4H
5.6		Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.1566, df = 13	p = 0.8770
8		Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.1592, df = 14	p = 0.8757
16		Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.9600, df = 14	p = 0.3533
32		Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 1.644, df = 9	p = 0.1346
Social interaction (M, %time with stranger)	5A	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.6283, df = 12	p = 0.5415
Social interaction (F, %time with stranger)	5B	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO	t = 0.9598, df = 10	p = 0.3598
Resident-intruder assay	5C	No	Mann–Whitney, two-tailed	Control vs Drd2^Pet1-^CKO	M–W U = 289.5	p = 0.6649
Tube test of social dominance	5E
Male		No	Mann–Whitney, two-tailed	Control vs Drd2^Pet1-^CKO	M–W U = 166	p = 0.0065
Female		No	Mann–Whitney, two-tailed	Control vs Drd2^Pet1-^CKO	M–W U = 253	p = 0.8123
Drd2-Pet1 neuron count	6A	Yes	Unpaired t test	Male vs female	t = 0.8160, df = 12	p = 0.4304
Soma size	6C	Yes	Unpaired t test	Male vs female	t = 1.021, df = 8	p = 0.3372
Gene expression	6D
Dmd		Yes	Unpaired t test	Male vs female	t = 0.9581, df = 7	p = 0.3699
Drd2		Yes	Unpaired t test	Male vs female	t = 1.514, df = 8	p = 0.1686
Gad2		Yes	Unpaired t test	Male vs female	t = 2.498, df = 8	p = 0.0370
Serpini1		Yes	Unpaired t test	Male vs female	t = 1.459, df = 7	p = 0.1879
%Gad2+ Drd2-Pet1 neurons	6E	Yes	Unpaired t test	Male vs female	t = 1.876, df = 8	p = 0.0975
% Drd2-Exon7/8+	7B	Yes	Unpaired t test	Control vs Drd2^Pet1-^CKO with Pet1 probe	t = 0.1291, df = 10	p = 0.8998
% Drd2-Exon7/8+	7B	Yes	One-way ANOVA	Control/Pet1 probe vs Drd2^Pet1-^CKO/ Pet1 probe vs Drd2^Pet1^-CKO /Cre probe	F_(2,19) = 0.1003	p = 0.9051
% Gad2 in Cre+ neurons	7D	Yes	Unpaired t test	Male vs female	t = 3.057, df = 8	p = 0.0157
Gad2 punctae per cell	7E	Yes	Unpaired t test	Male vs female	t = 1.768, df = 8	p = 0.1151
Nucleus area	7F	Yes	Unpaired t test	Male vs female	t = 0.9931, df = 8	p = 0.3497
Resting membrane potential	8A	Yes	Unpaired t test	Male vs female	t = 0.2113, df = 61	p = 0.8334
Membrane resistance	8B	Yes	Unpaired t test	Male vs female	t = −0.4084, df = 61	p = 0.6844
AP threshold	8B	Yes	Unpaired t test	Male vs female	t = 1.8197, df = 61	p = 0.0737
AP amplitude	8D	Yes	Unpaired t test	Male vs female	t = −1.0474, df = 61	p = 0.2990
AP duration	8E	Yes	Unpaired t test	Male vs female	t = −2.2583, df = 61	p = 0.0275
AHP amplitude	8F	Yes	Unpaired t test	Male vs female	t = 1.350, df = 61	p = 0.1821
Innervation densities	9C
DPGi		Yes	Unpaired t test	Male vs female	t = 1.285, df = 9	p = 0.2308
PAG		Yes	Unpaired t test	Male vs female	t = 0.2398, df = 9	p = 0.8158
mPOA		Yes	Unpaired t test	Male vs female	t = 0.1978, df = 9	p = 0.8476
DLG		Yes	Unpaired t test	Male vs female	t = 0.07798, df = 9	p = 0.9395
mHb		Yes	Unpaired t test	Male vs female	t = 0.6732, df = 9	p = 0.5178
PnC		Yes	Unpaired t test	Male vs female	t = 0.7901, df = 9	p = 0.4498
IC		Yes	Unpaired t test	Male vs female	t = 0.5350, df = 9	p = 0.6056
LL		Yes	Unpaired t test	Male vs female	t = 0.9100, df = 9	p = 0.3865
SOC		Yes	Unpaired t test	Male vs female	t = 0.9282, df = 9	p = 0.3775
CNC		Yes	Unpaired t test	Male vs female	t = 0.2997, df = 9	p = 0.7712

Statistical values are provided for behavioral analyses of Drd2^Pet1^-CKO mice and comparison of Drd2-Pet1 neuron properties in male versus female mice. Figure numbers are included to reference corresponding graphs. Statistical analyses were conducted in GraphPad Prism version 8.1.