Figure 4.

FENDRR directly targets miR-424-5p. Database starBase was used and it was found that miR-424-5p bore the targeted binding sites with FENDRR and (A) miR-424-5p was observed to be up-regulated in tumor tissue in the TCGA-COAD dataset. (B) qRT-PCR was performed to detect miR-424-5p in CRC cells and clinical tissue samples. (C) Dual-luciferase reporter assay was conducted to verify the targeting relationship between FENDRR and miR-424-5p. Afterward, si-FENDRR, oe-FENDRR and their negative controls were transfected into CRC cells. (D) qRT-PCR was carried out to assess miR-424-5p expression in each transfection group (* p < 0.05; ** p < 0.01).