Figure 5.

The antigen-specific cytokine responses of the splenocytes upon in vitro antigen recall. Spleens were harvested from mice 7 days after received a single dose of vaccine with or without miltefosine, and splenocytes were stimulated in vitro with the influenza vaccine for 19.5 h. (A) Schedule of influenza vaccine and spleen sample collection. (B) Number of IFN-γ secreting cells (left) and IL-4 secreting cells (right). MTF-VAC, miltefosine (0.2 mg) + vaccine group; VAC, vaccine only group; MTF, miltefosine (0.2 mg) only group; PBS, PBS only group. Data collected from 5–6 mice per group (2 independent experiments). * for p < 0.05; ** for p < 0.01; **** for p < 0.0001; calculated by two-way ANOVA followed by a Tukey’s multiple comparison test. Error bar indicates standard error of mean (SEM).