Table 3.
Approximate analytic sensitivity of commonly used molecular techniques.
| Technique | Approximate Analytic Sensitivity |
|---|---|
| Sanger Sequencing | 10–20% [14] |
| Restriction Fragment Length Polymorphism Analysis | 0.1–1% [15] |
| Pyrosequencing | 5% [14] |
| Next Generation Sequencing (germline) | 5–10% * |
| Next Generation Sequencing (somatic) | 5% ** |
| Real-Time PCR with Melt Curve Analysis | 1–10% [14] |
| Single-Stranded Conformational Polymorphism Analysis with Denaturing High Performance Liquid Chromatography | 1–5% [16] |
| Microsatellite Short Tandem Repeat Analysis by Capillary Electrophoresis | 1% *** |
| Amplification Refractory Mutation System Analysis | 0.05–0.1% [16] |
| Droplet Digital PCR | 0.25% [17] |
* varies based on read depth and pipeline. ** varies based on read depth and pipeline; somatic variant filtering tends to be more permissive. *** institutionally validated.