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. 2020 Dec 14;8(4):759. doi: 10.3390/vaccines8040759

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Western blot of the recombinant GspD secretin of Leptospira interrogans serovar Canicola (rGspDLC), expressed by E. coli BL21(DE3)pLysS transformants. Panel (A): Detection of an approximately 63 kDa band (arrow), using five sera from four naturally infected dogs (lanes 1–4) and one convalescent dog with leptospirosis (lane 5). Infection with serovar Canicola was confirmed by culture and MLST. Lane 6, anti-His-HRP positive control; lane 7, molecular weight marker. Panel (B): Detection of an approximately 63 kDa band (arrow), using the sera from eight hamsters experimentally infected with the virulent serovar Canicola strain LOCaS46 (lanes 2–9), and the infection was confirmed by culture. Sera from two non-infected hamsters were used as negative controls (lanes 1 and 10), where a band was not detected; lane 11, molecular weight marker.