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. 2020 Dec 14;8(4):759. doi: 10.3390/vaccines8040759

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemistry assay on renal tissue of experimentally infected hamsters (Mesocricetus auratus). Infection was performed by intraperitoneal inoculation with the virulent strain LOCaS46 of Leptospira interrogans serovar Canicola. Panels (A,C): Detection of leptospires using a 1:200 dilution of rabbit polyclonal hyperimmune antiserum against L. interrogans serovar Canicola. Panel (B): Detection of leptospires using a 1:25 dilution of the rabbit polyclonal antibody against the recombinant GspD secretin of L. interrogans serovar Canicola (rGspDLC). Panel (D): Renal tissue of a non-infected hamster used as a control.