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. 2020 Dec 15;12(12):3786. doi: 10.3390/cancers12123786

Figure 2.

Figure 2

Separation of human (tumor) and mouse (stroma) transcriptomes of BM18 and LAPC9 tumors. (A,B) Principal component analysis plot of the gene expression of the 500 most variable genes on all samples; BM18 human transcripts (A) and LAPC9 human (B) at intact, castrated and replaced (castrated + 24 h AR) conditions. (C,D) Expression values of AR direct target genes as detected by RNA-Seq (transcript per million (TPM) counts) in the BM18 (C) or LAPC9 (D) tumors as confirmation of the effective repression of AR downstream signaling by castration. Intact (n = 3), castrated (n = 4) and replaced (n = 3). (E,F) Principal component analysis plot of the gene expression of the 500 most variable genes on all samples, BM18 mouse (E) and LAPC9 mouse (F) at intact, castrated and replaced (castrated + 24 h AR) conditions. (G,H) Expression values of the prostate-specific bone metastasis signature (Ob-BMST) seven upregulated stroma signature genes, as detected by RNA-Seq (TPM normalized counts) in the mouse transcriptome of BM18 (E) or LAPC9 (F) tumors.