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. 2020 Dec 14;8(4):763. doi: 10.3390/vaccines8040763

Figure 1.

Figure 1

Deletion of the CD2v and UK genes from the ASFV-SY18 genome. (A) Schematic diagram representing the target site (s), the genes adjacent to the deletion sites and step-by-step generation of the double gene-deleted ASFV. The two recombinant transfer vectors designed to sequentially delete the CD2v gene at nt 73,369-74,451 and the UK gene at nt 184,341-184,631 of the ASFV-SY18 genome are shown. (B) ASFV-SY18-∆CD2v/UK amplification in alveolar macrophages (AM). ASFV-SY18-∆CD2v/UK- or ASFV-SY18-infected AM are shown in different fluorescence background (10×). (C) PCR analysis of ASFV-SY18-∆CD2v/UK stock. Viral DNA from ASFV-SY18-∆CD2v/UK or ASFV-SY18 infected AM were tested for the presence of CD2v or UK genes and the B646L (p72) gene was used as a positive control to detect ASFV genome, NC is a negative control.