Table 1.
Primers used in the study.
| Name | Sequence (5′–3′) | Description |
|---|---|---|
| CD2vd-LA-F | GGTACCCGGGAGCTCGAATTCGTCTAGTTATATATGTCGGTCA | Used to amplify the left arm |
| CD2vd-LA-R | GACTTTTCTCCGGCGACCCTTTATGAACATATGTTTTATA | |
| CD2vd-m-F | TATAAAACATATGTTCATAAAGGGTCGCCGGAGAAAAGTC | Used to amplify the EGFP marker gene |
| CD2vd-m-R | GGTTAAATAATTAATATATAGTTATCTAGATCCGGTGGATCCC | |
| CD2vd-RA-F | GGGATCCACCGGATCTAGATAACTATATATTAATTATTTAACC | Used to amplify the right arm |
| CD2vd-RA-R | GTCTGCAGAAGCTTCGAATTCGATATTTTGGCTATCATCGG | |
| CD2v-F | CACTAGCTACATGTGGAAAAGCAGG | Used to detect wild-type ASFV in CD2v-deleted ASFV purification |
| CD2v-R | GGGTAGATAATGGCGGGATATTG | |
| UKd-LA-F | CCCGGGAGCTCGAATTCGAAGCTTTTCACCTTTATGAAATGATC | Used to amplify the left arm |
| UKd-LA-R | GACTTTTCTCCGGCGACCCGCTAATAGTTACTATACAAAAATAG | |
| UKd-m-F | CTATTTTTGTATAGTAACTATTAGCGGGTCGCCGGAGAAAAGTC | Used to amplify the dsRed marker gene |
| UKd-m-R | GGATGGAGCGCATTAGGGATTACAGGAACAGGTGGTGGC | |
| UKd-RA-F | GCCACCACCTGTTCCTGTAATCCCTAATGCGCTCCATCC | Used to amplify the right arm |
| UKd-RA-R | GCCACGGCGATATCGGATCCAATAGAGTCATTATTTAATAATAGG | |
| UK-F | CCGCCTCCCCATTATTCTTC | Used to detect wild-type ASFV in UK-deleted ASFV purification |
| UK-R | GATGGAGCGCATTAGGGATCCC | |
| p72-F | GCTCGCCGAAGGGAATGGATAC | Used to detect ASFV DNA in recombinant virus isolation |
| p72-R | GGCCGACAAGATTATATTGG | |
| p72-F | CTGCTCATGGTATCAATCTTATCGA | Used in real-time PCR |
| p72-R | GATACCACAAGATCAGGCCGT | |
| eGFP-F | AGTCCGCCCTGAGCAAAGA | Used in real-time PCR to detect EGFP |
| eGFP-R | TCCAGCAGGACCATGTGATC |
(F) forward; (R) reverse; (RA) right arm; (LA) left arm; (m) marker; (d) deletion.