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. 2020 Dec 28;21(Suppl 21):534. doi: 10.1186/s12859-020-03885-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 14 — Reference image generation-based image fusion. a First original localized aligned particle image. b Second localized perfectly aligned particle image. c Blended overlay fused particle image, by scaling the intensities of the reference image. d Visualized the fused blended (overlay) image using the red channel for the reference particle image, the green channel for the aligned moving image, and yellow channel for the areas of similar intensity between the two images