Fig. 5.

Fully automated 2D side-view particle alignment using KLH dataset [12]. a Generated frontal view reference image using average binary particle object sizes. b Original side-view particle image (moving). c Perfect generated binary mask of b. d Unalignment images projection (references a and moving c). e Default image alignment (initial registration). f Optimizer adjustment and metric configuration-based image registration. g Image registration-based on increasing the maximum iteration number. h Image registration-based optimization and rigid transformation. i Image remigration using affine transform. j Original binary mask particle’s orientation. k Aligned binary mask particle’s orientation. l Final particle alignment result