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. 2020 Dec 28;21(Suppl 21):534. doi: 10.1186/s12859-020-03885-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 9 — Comparing the localized 2D particle image results before and after the perfect top-view particle centralized based particle image alignment. a, e Localized 2D top-view particle images from the KLH [12] and Apoferritin [11] datasets before the centralized particle image alignment. b, f Localized 2D top-view particle binary masks of a, e before the centralized particle image alignment. c, g Localized 2D top-view particle images of a, e after centralized based particle image alignment. (d, h) Localized 2D top-view particle masks of a, e after centralized based particle image alignment