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. 2020 Dec 7;117(51):32806–32815. doi: 10.1073/pnas.2020197117

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Copyright © 2020 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 4. — Rapid and robust DIA-based approach identifies GID substrates during recovery after glucose starvation. (A) Number of quantified proteins in wild type (WT), ΔGID4, and Gid2^K365A yeast cells during glucose starvation and recovery. (B and C) Bar graphs showing abundances (log2) of Fbp1 (B) and Mdh2 (C) proteins that are normalized to WT glucose (never starved) condition in WT, ΔGID4, and Gid2^K365A yeast cells during glucose starvation and recovery. (D) Heat map of z-scored protein abundances (log2) of the proteins which have the criteria of GID substrates.