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. 2019 Oct 10;221(5):729–738. doi: 10.1093/infdis/jiz521

Figure 2.

Figure 2.

Maternal immunization with ΔgD-2 protects 7- and 14-day-old pups from herpes simplex virus (HSV) disease. Female C57BL/6 mice were prime-boost subcutaneously immunized with 5 × 106 plaque-forming units (pfu) of ΔgD-2 or VD60 cell lysate control, mated 1-week postboost, and monitored for birth. Pups were then challenged intranasally at the indicated day of life with (A) 103 pfu of HSV-2(4674) (Day 1: ΔgD n = 5, VD60 n = 6; Day 7: ΔgD n = 16, VD60 n = 40; Day 14: ΔgD n = 22, VD60 n = 14) or (B) 105 pfu of HSV-1(B3x1.1) (Day 1: ΔgD n = 5, VD60 n = 17; Day 7: ΔgD n = 48, VD60 n = 45; Day 14: ΔgD n = 23, VD60 n = 14). Statistical significance was determined by Kaplan-Meier (Day 7, 14) and analysis of variance with Tukey’s multiple comparison test (Day 1). Significance is shown relative to VD60 lysate control (*, P < .05; ***, P < .001).