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. 2020 Dec 7;117(51):32566–32573. doi: 10.1073/pnas.2011501117

Fig. 1.

Fig. 1.

gp120 V2 binding to α4β7 activates CD4+ T cells. (A) Flow cytometric analysis of CD25/-Ki67 double positive primary CD4+ T cells cultured for 96 h in the presence of wells coated with anti-CD3 alone or anti CD3 in combination with: MAdCAM, 92Th023cV2, 92TH023 + a control IgG mAb, 92TH023cV2 + anti α4 mAb (2B4), 92TH023cV2 + anti gp120 V2 mAb (CH58). The y axis indicates the % cells expressing both CD25 and Ki67. Purified bulk CD4+ T cells were obtained from eight independent donors. *P < 0.05 **P < 0.01 (two-tailed parametric paired t test). (B) DI (by CFSE dye dilution) of primary CD4+ T cells isolated from seven independent donors and stimulated with ligands as in A and also with anti-CD3 + MAdCAM. *P < 0.05, **P < 0.01 (two-tailed parametric paired t test). (C) A244 gp120 binding to α4β7 mediates proliferation of CD4+ T cells. Flow cytometric determination of average DI in primary CD4+ T cells from three independent donors cultured for 96 h in the presence of wells coated with anti-CD3 alone or anti-CD3 in combination with A244 gp120, A244 gp120 + a control IgG mAb, A244 + a V2 mAb (CH58), or A244 + CD4 binding site mAb VRC01. **P < 0.01 (two-tailed parametric paired t test).