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. 2020 Dec 8;117(51):32464–32475. doi: 10.1073/pnas.2005868117

Fig. 3.

Fig. 3.

LncMyoD is required for myogenic lineage progression. (A, Left) Single fibers were isolated and cultured for 3 d under differentiation condition after siRNA KD and stained for Syn4 and MyoD/MyoG. Syn4, MyoD double-positive cells are highlighted by arrowheads. Syn4, MyoG double-positive cells are highlighted by arrows. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (Scale bar: 50 μm.) (A, Right) Quantification of MyoD+/MyoG+ cells per fiber and percentage of Syn4+ cells that are MyoD+/MyoG+ in Ctrl and LncMyoD KD fibers. NS, not significant. ***P < 0.001. (B, Left) FACS-sorted SCs were cultured for 3 d under differentiation condition after siRNA KD and stained for Ki67 and MyoD/MyoG. The cell nuclei were stained with DAPI (blue). (Scale bar: 100 μm.) (B, Right) Quantification of the percentage of MyoD+ and MyoG+ cells in Ctrl and LncMyoD KD SCs. NS, not significant. ***P < 0.001. (C, Left) Morphology of FACS-sorted SCs cultured 4 d under differentiation condition after siRNA treatment. (C, Right) Fusion index calculated from FACS-sorted SCs cultured 4 d under differentiation condition after siRNA treatment. **P < 0.01.