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. 2020 Dec 8;117(51):32464–32475. doi: 10.1073/pnas.2005868117

Fig. 7.

Fig. 7.

LncMyoD is essential for the establishment of the myogenic program in 10T1/2 fibroblasts. (A) WT and LncMyoD KO 10T1/2 fibroblast clones were induced to differentiate for 8 d after transfection of the indicated MyoD adenovirus. Cells were then fixed to visualize myotube formation. The cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (B) Fusion index calculation of WT and LncMyoD KO clones for MyoD adenovirus treatment. *P < 0.05, ***P < 0.001. (C) WT and LncMyoD KO 10T1/2 fibroblast clones were induced to differentiate for 8 d after transfection of the indicated LncMyoD plasmid and MyoD adenovirus. Cells were then fixed to visualize myotube formation. The cell nuclei were stained with DAPI (blue). (D) Fusion index calculation of WT and LncMyoD KO clones for MyoD adenovirus and LncMyoD-containing plasmid treatment. NS, not significant.