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. 2020 Dec 1;117(51):32413–32422. doi: 10.1073/pnas.2010292117

Fig. 1.

Fig. 1.

Correlating actin speed with force on talin. (A) Schematic of talin tension sensor (TS) and talin C-terminal control sensor (CTS). (B) Heat maps for simultaneous talin tension-sensor FRET index and actin speed in large lamellar adhesions in untransfected cells (Left) and lamellipodial adhesions in RacV12-expressing cells (Right). (Scale bar, 10 µm.) (C) Binned pixelwise correlations of FRET vs. actin speed (mean ± 95% confidence interval, TS n = 49, CTS n = 28, TS RacV12 n = 14, CTS RacV12 n = 12, cells from 10 independent experiments). ns, not statistically significant. (D) Quantification of average actin speed on a per-cell basis and (E) normalized FRET index for cells transfected with talin TS or CTS during spreading on fibronectin-coated glass. FRET index is normalized to the average value of the control sensor. LM: lamellar, LP: lamellipodial (box plots indicate median and quartiles, dots indicate single-cell averages, for data shown in C, **P < 0.01, ***P < 0.001, ****P < 0.0001, one-way ANOVA with Tukey’s post hoc).