Skip to main content
. 2020 Dec 1;117(51):32413–32422. doi: 10.1073/pnas.2010292117

Fig. 4.

Fig. 4.

Vinculin transmits flow-independent force. (A) Western blot and quantification for vinculin after infection with nontargeting (NT) or vinculin guide RNA, with tubulin loading control (n = 4 experiments, ****P < 0.0001, two-sided t test). (B) Representative heat maps of talin FRET index and actin speed. (Scale bar, 10 µm.) (C) Average actin speed and (D) normalized FRET index per cell after vinculin depletion (box plots indicate median and quartiles, dots indicate single-cell averages, NT n = 31, VCL n = 33, CTS n = 22 cells per group from three independent experiments, *P < 0.05, ***P < 0.001, one-way ANOVA with Dunnett’s post hoc test). (E) Normalized FRET index for Talin TS and CTS before and after addition of 0.1% PFA, with vinculin (VCL) or nontargeting control guide RNA (NT) (box plots indicate median and quartiles, dots indicate single-cell averages, TS-NT n = 15, TS-VCL n = 18, CTS-NT n = 17, TS-NT-RacV12 n = 19, TS-VCL-RacV12 n = 15, CTS-NT-RacV12 n = 17, matched cells from two independent experiments, *P < 0.05, ***P < 0.001, two-way ANOVA with Sidak’s post hoc test).