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. 2020 Dec 1;117(51):32413–32422. doi: 10.1073/pnas.2010292117

Fig. 5.

Fig. 5.

Force on vinculin does not correlate with actin flow. (A) Schematic of the vinculin tension sensor (VTS) and tailless control sensor (VTL). (B) Representative heat maps of actin speed and vinculin FRET index normalized to VTL for lamellar adhesions in WT 3T3 cells. (Scale bar: 10 μm.) (C) Quantification of average per-cell actin speed and (D) vinculin TS and VTL normalized FRET index for lamellar adhesions during spreading on fibronectin-coated glass (box plots indicate median and quartiles, dots indicate single-cell averages, VTS n = 27, VTL n = 16, ***P < 0.001). (E) Binned pixelwise correlations of vinculin sensor FRET vs. actin speed (mean ± 95% confidence interval).