. Author manuscript; available in PMC: 2021 Jun 1.

Published in final edited form as: Am J Transplant. 2020 Jan 20;20(6):1691–1702. doi: 10.1111/ajt.15761

Table 2.

Purity, yield and ex vivo expansion of Treg from normal PBMC or leukapheresis products (n=3)

Animal #		Total cell number	MACS-isolated CD4⁺CD25^hi Treg			Expanded CD4⁺CD25^hiFoxp3^hiTreg^†
Animal #		(x10⁶)	Purity (%)	Yield (%)	(x10³)	Fold change (x10³)	Purity (%) ^*
RM51	PBMC	28.8	90.9	0.55	160	540	97.8
RM51	Leukapheresis	31.5	96.6	0.86	270	319	96.0
RM120	PBMC	23.3	92.5	0.46	110	462	97.7
RM120	Leukapheresis	42.0	95.7	0.59	250	370	96.7
RM238	PBMC	30.2	80.4	0.79	240	299	93.6
RM238	Leukapheresis	36.3	92.3	0.83	300	304	93.1
(Mean±SD)	PBMC	27.4 ± 3.6	87.9 ± 6.6	0.60 ± 0.17	170 ± 70	434 ± 123	96.4 ± 2.4
(Mean±SD)	Leukapheresis	36.6 ± 5.3	94.9 ± 2.3	0.76 ± 0.15	270 ± 30	331 ± 35	95.3 ± 1.9
	p value	0.200	0.116	0.181	0.047	0.257	0.101

Determined by flow cytometry

^†

Cells were expanded for 24 days