Table 1.

Characteristics of 72 studies included to explore the diagnostic value of microRNAs in asthma.

Study ID	Region	No. of cases/controls	Diagnosis criteria	Differentially expressed miRNAs	Specimen sources	Detection method	NOS
Children
Ibrahim et al.50	Egypt	100/50	GINA	196a2	Serum	qRT-PCR	8
Bartel et al.63	Germany	18/9	Hospital	92b, 210, 34a	ASMCs	qRT-PCR	8
Karam and Abd Elrahman61	Egypt	100/100	GINA	155, let-7a	Plasma	qRT-PCR	8
Li et al.60	China	23/15	Hospital	378	Peripheral blood, lung tissue	qRT-PCR	8
Liu et al.58	China	180/180	ISAAC	155	Serum	qRT-PCR	8
Nasser et al.57	Egypt	30/20	GINA	15a	Serum	qRT-PCR	8
Dong et al.71	China	150/50	GINA	27b-3p	BECs	Microarray, qRT-PCR	8
Hammad et al.30	Egypt	27/21	GINA	21, 146a	Plasma	qRT-PCR	8
Tian et al.69	China	100/100	GDPB	1	Blood	qRT-PCR	8
Tian et al.69	China	80/80	CGBS	126	Serum	qRT-PCR	8
Zhang et al.67	China	18/15	Hospital	192	CD4+ T cellsa, plasma	qRT-PCR	8
Zhang et al.64	China	52/26	GINA	29c	CD4+ T cellsa	Microarray, qRT-PCR	8
Hu et al.80	China	124/382	Hospital	149	PBMCs	qRT-PCR	8
Dong et al. 93	China	62/62	GINA	625-5p, 513a-5p, 22-3p	Plasma	Microarray, qRT-PCR	8
Elbehidy et al. 92	Egypt	95/80	GINA	21	Serum	qRT-PCR	8
Liu et al. 88	China	50/50	GINA	125b	Sputum	qRT-PCR	8
Midyat et al. 85	USA	95/96	GINA	let-7e, 98, 497	Blood	qRT-PCR	8
Zhang et al. 82	China	30/30	Hospital	146a	Plasma	qRT-PCR	8
Sawant et al. 95	USA	8/8	Hospital	21	Serum	qRT-PCR	7
Liu et al. 109	China	6/6	GINA	483-3p, 221	Blood	Microarray, qRT-PCR	8
Adult
ElKashef et al. 51	Egypt	30/30	Hospital	21, 155	Serum	qRT-PCR	8
Liang and Tang 49	China	772/441	GINA	155	CD4+ T cellsb	qRT-PCR	7
Ye et al. 48	China	180/90	GINA	125a	Plasma	qRT-PCR	8
Fang et al. 62	Switzerland	5/5	Hospital	21-5p	ASMCs	qRT-PCR	6
Huang et al. 22	China	70/34	GINA	let-7a-5p	Peripheral blood	Microarray, qRT-PCR	8
Liang et al. 59	China	50/15	Hospital	218-5p	BECs	qRT-PCR	8
Qiu et al. 75	China	52/45	CGBS	17	CD4+ T cellsb	Microarray, qRT-PCR	8
Rodrigo-Muñoz et al. 55	Spain	173/53	GEMA	185-5p, 320a, 1246, 144-5p	Serum	NGS, qRT-PCR	8
Tsai et al. 54	China	86/38	GINA	10a-5p, 146a-5p	BECs, serum	NGS, qRT-PCR	7
Wu et al. 53	China	53/47	GINA	1165-3p	Serum	qRT-PCR	8
Zhao et al. 52	China	80/80	GINA	125b	Serum	qRT-PCR	8
Daniel et al. 72	USA	16/28	NHLBI	155	CD4+ T cellsb	Microarray, qRT-PCR	8
Faiz et al. 31	Australia	16/39	Hospital	146a	Bronchial biopsy	Microarray, qRT-PCR	7
Huang et al. 70	China	35/15	GINA	199a-5p	Plasma, sputum	qRT-PCR	8
Tang et al. 26	China	49/22	ATSG	200b/c, 21, 1268, 663a, let-7a/b, 30a, 99a, 27a, 133a, 155, 24	BALF	qRT-PCR	8
Wang et al. 68	China	82/80	CGBS	21	Serum	qRT-PCR	8
Zhang et al. 66	China	25/22	GINA	221	BECs	qRT-PCR	8
Zhang et al. 65	China	77/36	Hospital	221-3p	BECs, sputum, plasma	qRT-PCR	8
Chen et al. 81	China	20/20	Hospital	98	PBMCs	qRT-PCR	8
Kärner et al. 79	Swiss	NA	GINA	323-3p	PBMCs	qRT-PCR	8
Lacedonia et al. 78	Italy	13/46	GINA	145, 338	Serum, sputum	qRT-PCR	8
Malmhäll et al. 111	Sweden	16/13	Hospital	155	Sputum	qRT-PCR	8
Milger et al. 76	Germany	46/21	Hospital	21-5p,15a-5p,27a-3p,29c-3p,223-3p,425-5p, 342-3p	Plasma	qRT-PCR	8
Qiu et al. 75	China	30/25	Hospital	371, 138, 544, 145, 214	CD3+ T cellsb	Microarray, qRT-PCR	8
Sun et al. 74	Italy	9/17	Hospital	101, 19a	ASMCs	qRT-PCR	7
Yin et al. 73	China	46/10	Hospital	34, 449	Serum	qRT-PCR	8
Cai et al. 94	China	28/28	GINA	194, 375	Plasma	qRT-PCR	8
Fan et al. 91	China	59/49	GINA	145	CD4+ T cellsb	qRT-PCR	8
Huo et al. 90	China	72/35	Hospital	181b-5p, 181a-5p	Bronchial brushing biopsy, plasma	Microarray, qRT-PCR	8
Li et al. 89	China	10/10	Hospital	31	Plasma, BECs	qRT-PCR	8
Luo et al. 87	China	20/25	Hospital	98	PBMCs	qRT-PCR	8
Maes et al. 86	Belgium	76/20	GINA, ATSG	629-3p, 223-3p, 142-3p	Sputum	qRT-PCR	8
Panganiban et al. 84	USA	35/44	Hospital	16, 223, 148, 146a, 299-5p, 570, 150	Plasma	qRT-PCR	8
Pietrusinńska et al. 83	Poland	3/6	GINA	224, 339-5p, 382	Plasma	qRT-PCR	7
Haj-Salem et al. 96	Canada	15/9	ATSG	19a	BECs	qRT-PCR	8
Newcomb et al. 29	USA	29/9	GSARP	let-7f	CD4+ T cellsb	qRT-PCR	8
Martinez-Nunez et al. 102	UK	15/13	Hospital	18a, 27a, 128, 155	BECs	Microarray, qRT-PCR	8
Perry et al. 101	USA	18/9	ATSG	221	ASMCs	qRT-PCR	8
Rijavec et al. 100	Slovenia	24/12	GINA	let-7a	Bronchial biopsy	qRT-PCR	8
Roff et al. 99	USA	10/10	Hospital	570-3p	BECs, serum	qRT-PCR	8
Simpson et al. 98	USA	24/8	Hospital	19a	CD4+ T cellsc	qRT-PCR	8
Suojalehto et al. 21	Finland	117/33	GINA	18a, 126, 155, let-7e, 224, 498, 187, 87, 143, 886-2p	Nasal mucosa	qRT-PCR	8
Wu et al. 97	China	35/12	GINA	21, 126	BECs	qRT-PCR	8
Levänen et al. 27	Sweden	10/10	Hospital	let-7a, 21, 658, 24,26a, 99a, 200c, 1268	BALF	Microarray, qRT-PCR	8
Nicodemus-Johnson et al. 103	USA	22/32	Hospital	148b	BALF	qRT-PCR	7
Jardim et al. 28	USA	16/16	ATSG	487b, 181c, let-7f	BECs	Microarray, qRT-PCR	7
Panganiban et al. 108	USA	10/10	Hospital	1248, 26a, let-7a, let-7d	Serum	Microarray, qRT-PCR	8
Solberg et al. 107	USA	35/12	Hospital	34c-5p, 34b-5p, 449a, 449b-5p	BECs	Microarray, qRT-PCR	8
Tsitsiou et al. 106	UK	16/8	ATSG	146a, 146b, 28a-5p	CD4+ T cellsb, CD8+ T cellsb	Microarray, qRT-PCR	8
Yamamoto et al. 105	UK	7/4	Hospital	192	PBMCs	qRT-PCR	7
Zhang et al. 104	China	50/20	CGBS(2003)	155	CD4+ T cellsb	qRT-PCR	8
Williams et al. 110	UK	8/8	Hospital	140, 449, 200c, 30b/c/d, 93, 223, 191, 320, 342,142-3p	Airway biopsies	qRT-PCR	7

^aFrom peripheral blood.

^bFrom PBMCs.

^cFrom BALF.

ASMCs, airway smooth muscle cells; BALF, bronchoalveolar lavage fluid; BECs, bronchial epithelial cells; PBMCs, peripheral blood mononuclear cells.