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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Cell Microbiol. 2020 Aug 13;22(11):e13244. doi: 10.1111/cmi.13244

Figure 3. Biosynthetic trafficking to the lysosome.

Figure 3.

TbVps24 silencing was induced (12 hr) and pulse chase radiolabeling was performed followed by immunoprecipitation with anti-TbCatL (A & B) or anti-p67 (C & D) at the indicated chase time points. A. TbCatL samples were fractionated by SDS-PAGE and a representative phosphorimage is presented. Cell and media samples are from the same gel/image, but were digitally separated and individually contrasted to allow ready visualization of the media samples. B. Conversion of precursor forms (P+X) to mature TbCatL (M) was quantified and is presented as mean ± std. dev. (n=3). Recovery of media samples is offset with an expanded scale. *** p<0.001. C. Likewise, p67 immunoprecipitates were fractionated and a representative phosphorimage is presented. D. Conversion of the gp100 to gp150, and subsequently to gp42 and gp32, was quantified (mean ± std. dev., n=3).