The manufacturer supplied positive control (PLC-δ1 PH domain, 0.5 μg/ml), recombinant TbZFP (negative control, 3 μg/ml) and recombinant TbVps24 (3 μg/ml) were incubated with phospholipid strips. After washing blots were probed with anti-GST (positive control) or anti-His antibody (recombinant T. brucei proteins). Representative Li-Cor images are presented. LPA, lysophosphatidic acid; LPC, lysophosphatidylcholine; PI, phosphatidylinositol; PE, phosphatidylethanolamine; PC, phosphatidylcholine; S1P, sphingosine-1-phosphate; PA, phosphatidic acid; PS, phosphatidylserine.