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. 2020 Nov 26;11:577302. doi: 10.3389/fphar.2020.577302

FIGURE 1.

FIGURE 1

Docosahexaenoic acid suppresses (12-O-tetradecanoylphorbol-13-acetate) TPA-induced Urokinase-type plasminogen activator receptor (uPAR) expression in ECV304 cells. (A–C) TPA induced the expression of uPAR in a time-dependent manner in ECV304 cells. Cells were treated with 100 nM TPA for 0–16 h. The uPAR protein level (A,B) and promoter activity (C) were measured by western blotting and luciferase activity assay, respectively. (D–G) Cells were pretreated with Docosahexaenoic acid (25, 50, and 100 μM) for 1 h, followed by incubation with 100 nM TPA for 4 or 16 h. The uPAR mRNA level (D), protein level (E,F), and promoter activity (G) were measured by real-time PCR, western blotting, and luciferase activity assay, respectively. *p < 0.05 versus control; **p < 0.05 versus TPA treatment alone. Data represent the mean ± SD of triplicate measurements.