FIGURE 5.

Role of HO-1 on the inhibition of (12-O-tetradecanoylphorbol-13-acetate) TPA-induced cell invasion. (A,B) Cells (10⁵) transfected with si-HO-1 or si-con were incubated with 100 nM TPA in the presence or absence of 100 μM Docosahexaenoic acid in a BIOCOAT^™ Matrigel apparatus for 48 h (C,D) Cells (10⁵) were incubated with 100 nM TPA in the presence or absence of 20 μM FePP in a BIOCOAT^™ Matrigel apparatus for 36 h. After incubation, the cells that invaded the undersurface of the chambers were counted using a phase contrast light microscope after staining with Diff-Quick stain. *p < 0.05 versus control; **p < 0.05 versus TPA treatment alone in the si-con group; ***p < 0.05 versus TPA and Docosahexaenoic acid treatments in the si-con group. Data represent the mean ± SD of triplicate measurements.