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. Author manuscript; available in PMC: 2021 May 18.
Published in final edited form as: Nature. 2020 Nov 18;588(7837):308–314. doi: 10.1038/s41586-020-2915-3

Extended Data Figure 5. Assessment of Ldlrad3 or LDLRAD3 surface expression and VEEV infection in gene-edited cell lines and primary cells.

Extended Data Figure 5.

a. Control and ΔLdlrad3 293T, 3T3, HeLa, and hCMEC/D3 cells were assessed for Ldlrad3 or LDLRAD3 surface expression (left panels) and SINV-VEEV-GFP TrD infection via GFP expression by flow cytometry (right panels). Two independent Ldlrad3 or LDLRAD3 gene-edited cell lines were generated (sgRNAs #1 and #2) and evaluated. Mean ± SD of three experiments (Ldlrad3 surface expression, n = 6; VEEV infection, n = 9; one-way ANOVA with Dunnett’s post-test: **** P < 0.0001). b. Primary cell lines (CADMEC, HDF, HPBM, and HPBT) were assessed for LDLRAD3 surface expression using anti-Ldlrad3 polyclonal serum (left panels, red). Cells were inoculated with SINV-VEEV-GFP and assessed for infection via GFP expression by flow cytometry (right panels, orange). The population of infected cells are indicated for each cell line. Data are representative of two or three experiments.