. Author manuscript; available in PMC: 2021 Nov 25.

Published in final edited form as: J Med Chem. 2020 Nov 11;63(22):14087–14117. doi: 10.1021/acs.jmedchem.0c01669

Table 3.

Inhibitory Activity of Zone 5-Modified Triazoloquinazolinone Derivatives at the Plk1 PBD (IC₅₀ Values Are n = 3 Unless Noted in Parentheses) and Microsomal Half-Life, PAMPA Assays, and Aqueous Solubility^a


Cmpd	R² =	R³ =	IC50 (μM, ELISA)	t_1/2 (min, RLM)	PAMPA (1e-6 cm/s)	Solub. (μg/ml)
21	Pr	H	1.03 ± 0.08 (9)	>30	37	21.3
35	Pr		1.63 ± 0.05	ND	ND	Insol.
36	CH₃		1.20 ± 0.03	ND	ND	ND
37	Pr		1.83 ± 0.04	ND	ND	ND
38	Pr		1.67 ± 0.06	ND	ND	8.7
39	Pr		>50	ND	ND	ND

ND, not determined