Table 7.

Effects of Different Instruments, Columns, Column Temperatures, Flow Rates, Volume and pH on RRTs (n = 3)

	Effects Factor	RPZ	PPZ	IPZ	OPZ(EOPZ)	LPZ
RSD%	Chromatographic Columns	2.70	3.96	2.75	–	4.75
	HPLC Instruments	2.65	1.52	1.34	–	0.89
	Column Temperatures	2.35	1.20	0.60	–	0.68
	Flow Volume of Mobile Phase	1.14	1.07	1.01	–	2.04
	Flow Rate of Mobile Phase	0.27	0.24	0.48	–	0.44
	pH Value of Mobile Phase	15.48	2.34	13.72	–	3.07
	χ	0.69	1.29	1.79	–	1.98
	S	0.003	0.005	0.007	–	0.008
	χ±3S	0.69±0.009	1.29±0.015	1.79±0.021	–	1.98±0.024