Figure 5:

Speculative model

Upon polysome disassembly, G3BP-USP10 complexes bind to dissociated 40S subunits. Prior to its dissociation from G3BP, USP10 mediates deubiquitination of specific ribosomal proteins to prevent their degradation through the lysosome. Binding of UBAP2L and caprin1 to G3BP leads to increased valency, which promotes LLPS and subsequent SG formation. The schematic was created in ©BioRender (biorender.com); it is not time-resolved and does not resemble actual sizes and ratios.