Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 23;218(2):e20201217. doi: 10.1084/jem.20201217

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Wang et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 8. — ZFP91 promotes autophagy-mediated T reg cell metabolism and homeostasis. (A and B) Flow cytometric analysis of p-S6 (A; n = 3 mice for each group) and c-Myc (B; n = 4 mice for each group) expression in Zfp91^+/+Foxp3-Cre, Becn1^fl/flFoxp3-Cre, Zfp91^fl/flFoxp3-Cre, and Zfp91^fl/flBecn1^fl/flFoxp3-Cre (DKO) T reg cells stimulated for 4 h with antibodies against CD3 and CD28. (C and D) ECAR of Zfp91^+/+Foxp3-Cre, Becn1^fl/flFoxp3-Cre, Zfp91^fl/flFoxp3-Cre, and Zfp91^fl/flBecn1^fl/flFoxp3-Cre (DKO) T reg cells stimulated for 3 h with antibodies against CD3 and CD28. (E and F) Flow cytometry analyzing Foxp3 expression (E) or cytokine expression (F) in Zfp91^+/+Foxp3-Cre, Becn1^fl/flFoxp3-Cre, Zfp91^fl/flFoxp3-Cre, and Zfp91^fl/flBecn1^fl/flFoxp3-Cre (DKO) T reg cells activated in vitro with anti-CD3, anti-CD28, and IL-2 for 24 h (n = 4 mice for each group). Data are representative of three independent experiments and are presented as mean ± SEM. ns, not statistically significant; **, P < 0.01. Two-tailed Student’s t test. 2-DG, 2-deoxy-D-glucose; MFI, mean fluorescence intensity. mph, milli-pH units.