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. 2020 Jul 26;19(1):74–86. doi: 10.1111/pbi.13441

Figure 3.

Figure 3

The CPK28/CPK29 module confers stress tolerance without causing a growth penalty in the high‐biomass crop tobacco. (a) Schematic overview of vectors used for transformation of tobacco. (b) Representative photographs of tobacco plants after five weeks (top view) and after seven weeks (side view) of growth. Scale bars: 10 cm. (c) Biomass production under control conditions. Leaf biomass (FWP: fresh weight per plant) was measured after 7 weeks of growth. (d) Determination of the dry weight (DWP) of the same material. (e) Seed yield. (f) Biomass production (FW) under osmotic stress conditions relative to the unstressed control. (g) Survival rate under drought stress. (h) Representative image of a wild‐type plant, two N‐28 × 29 lines, and the two parental lines (N‐28 and N‐29) after one week without watering. Asterisks indicate statistically significant differences between transgenic lines and the wild type as determined by one‐way ANOVA followed by Dunnett's multiple comparison (*P < 0.05, **P < 0.01). All data are presented as means ± SD from two (c–e) or three (f, g) independent experiments (c–e: n = 5‐6 biological replicates, each with 10 plants per line; f: n = 9 biological replicates, each with 10 plants per line; and g: n = 36 biological replicates, each with 3 plants per line).