Figure 5.
Equine enteroid culture. (a) Time course crypt culture 0–120 h postplating. (b) Whole mount imaged enteroid with fluorescently labeled enteroendocrine cells (red), proliferative cells (green), and general nuclear stain (blue). Outwardly budding crypts are visible peripherally with formation of a pseudolumen centrally. Three-dimensionality creates multiple visual planes with cells visible both in and out of focus. (c) Formalin-fixed, paraffin-embedded, sectioned, and hematoxylin- and eosin-stained 7-day cultured enteroid.