Skip to main content
. 2020 Dec 14;16(12):e1009096. doi: 10.1371/journal.ppat.1009096

Fig 2. Induction of pro-inflammatory cytokines by ΔLprG and BCG vaccines in C3HeB/FeJ mice.

Fig 2

A) Vaccination regimen. C3HeB/FeJ mice were vaccinated with 100uL of O.D. 1.0 log-phase culture of either BCG Serum Statens Institute (SSI) or H37RvΔlprG-rv1410c (ΔLprG) subcutaneously in the left flank. Serum for Luminex cytokine analysis was collected on days 1 and 7 after vaccination. PBMC were collected at weeks 2, 6, and 9 post-vaccination. B) Heat-map showing median log2 fold-change of serum cytokine levels as compared to naïve mice in BCG and ΔLprG vaccinated mice on days 1 and 7. C-H) Individual cytokine levels in serum on days 1 and 7; bar represents median values;* p<0.05; ** p<0.01, Mann-Whitney U test (BCG vs. ΔLprG). LLOQ represents lower limit of quantification. Luminex assays were performed twice with 5–8 mice per group. Data is representative of individual experiments.