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. 2020 Dec 14;16(12):e1009246. doi: 10.1371/journal.pgen.1009246

Fig 3. Tn-seq reveals coat genes that are important for sporulation in the absence of ssdC.

Fig 3

(A) Scatterplot showing fold-reduction of transposon insertions in ΔssdC (bCR1565) relative to wild-type (WT) cells (BDR2413), with corresponding p-values. Spore coat genes with high fold-reduction in ΔssdC compared to WT cells and low p-value are labelled and coloured cyan. (B) Sporulation efficiency of mutant strains ΔsafA (bBK33), ΔspoVID (bBK3), ΔssdC (bBK28), ΔssdC ΔsafA (bBK48) and ΔssdC ΔspoVID (bBK43) as a percentage of wild-type (BDR2413, WT). Error bars represent standard deviation of three biological replicates. (C) Tn-seq profiles at the safA and spoVID genomic loci of wild-type (BDR2413, WT) and ΔssdC (bCR1565) cells, following 24 h of growth and sporulation in exhaustion medium. Height of vertical lines represents number of transposon-sequencing reads at each position. Shaded regions highlight the significant reduction in sequencing reads at safA and spoVID loci.