Table 3. Primary antibody details.
| Antibody | Specificity | Dilution | Clone | Source |
|---|---|---|---|---|
| MOG | Myelin oligodendrocyte glycoprotein | 1:50 | Mouse (IgG2a) Y10 | Reynolds, Imperial |
| MBP | Myelin basic protein | 1:500 | Rabbit polyclonal | Merck |
| NFil | 200 kD neurofilament protein | 1:500 | Mouse (IgG1) NE14 | Merck |
| SMI32 | Dephosphorylated neurofilament proteins | 1:500 | Mouse (IgG1) SMI32 | BioLegend |
| Caspr1 | Contactin-associated protein 1 | 1:300 | Rabbit EPR7828 | Abcam |
| Caspr1 | Contactin-associated protein 1 | 1:500 | Mouse (IgG1) K65/35 | Neuromab |
| Pan-Nav | Voltage-gated sodium channels | 1:100 | Mouse (IgG2a) K58/35 | Neuromab |
| Kv 1.2 | Voltage-gated potassium channel 1.2 | 1:100 | Mouse (IgG2a) K14/16 | Neuromab |
| HLA-DR | MHC class II human leucocyte antigen DR | 1:200 | Mouse TAL.1B5 | Dako Agilent |
| IBA1 | Ionized calcium binding adaptor molecule 1 | 1:500 | Rabbit polyclonal | Wako |
| GFAP | Glial fibrillary acidic protein | 1:500 | Rabbit polyclonal | Dako Agilent |
| Calbindin1 | Calbindin1 (Purkinje neurons) | 1:500 | Mouse (IgG1) CB-955 | Merck |
| NeuN | Neuron specific nuclear protein | 1:500 | Mouse (IgG1) A60 | Merck |
Antigen, specificity, concentration, clone, and source of the primary antibodies used in this project.