Table 3.
Univariate and multivariate COX regression analysis of m6A regulatory genes for CRC patients' OS and DFS.
| Characteristics | OS | DFS | ||||||
|---|---|---|---|---|---|---|---|---|
| Univariate | Multivariate | Univariate | Multivariate | |||||
| HR (95% CI) | p | HR (95% CI) | p | HR (95% CI) | p | HR (95% CI) | p | |
| Age (>65 vs ≤65) | 2.5 (1.68-3.72) | 0.000 | 2.61 (1.08-6.28) | 0.033 | 1.05 (0.72-1.53) | 0.811 | 1.09 (0.70-1.71) | 0.700 |
| Sex (male vs female) | 1.07 (0.75-1.53) | 0.707 | 1.35 (0.91-1.98) | 0.132 | ||||
| Tumor stage | ||||||||
| I | 1 | 1 | 1 | |||||
| II | 1.62 (0.71-3.68) | 0.249 | NA (0-inf) | 0.998 | 2.22 (0.99-4.99) | 0.054 | 2.16 (0.38-12.47) | 0.388 |
| III | 3.07 (1.37-6.88) | 0.006 | NA (0-inf) | 0.997 | 3.62 (1.61-8.12) | 0.002 | 3.17 (0.45-22.27) | 0.246 |
| IV | 7.96 (3.55-17.86) | 0.000 | NA (0-inf) | 0.997 | 8.45 (3.66-19.48) | <0.001 | 1.08 (0.07-16.48) | 0.959 |
| T stage | ||||||||
| T1 | 1 | 1 | 1 | |||||
| T2 | 0.83 (0.18-3.92) | 0.816 | NA (0-inf) | 0.999 | 0.92 (0.2-4.25) | 0.914 | 1.16 (0.14-9.88) | 0.890 |
| T3 | 1.92 (0.47-7.8) | 0.363 | NA (0-inf) | 0.999 | 2.05 (0.5-8.36) | 0.315 | 1.03 (0.09-11.36) | 0.980 |
| T4 | 5.92 (1.4-25.06) | 0.016 | NA (0-inf) | 0.999 | 6.19 (1.45-26.54) | 0.014 | 3.01 (0.27-34.02) | 0.370 |
| N stage | ||||||||
| N0 | 1 | 1 | 1 | |||||
| N1 | 1.77 (1.13-2.78) | 0.013 | 0.11 (0.02-0.63) | 0.013 | 1.8 (1.14-2.84) | 0.011 | 0.86 (0.26-2.86) | 0.805 |
| N2 | 4 (2.63-6.08) | <0.001 | 0.45 (0.09-2.32) | 0.338 | 3.58 (2.27-5.64) | <0.001 | 1.81 (0.54-6.05) | 0.334 |
| M stage (M1 vs M0) | 4.28 (2.85-6.42) | <0.001 | 3.59 (2.26-5.68) | <0.001 | 6.97 (0.78-62.58) | 0.083 | ||
| Site | ||||||||
| Abdomen | 1 | 1 | ||||||
| Colon | 0.42 (0.06-3.02) | 0.389 | 0.35 (0.05-2.54) | 0.301 | ||||
| Rectum | 0.32 (0.04-2.42) | 0.267 | 0.36 (0.05-2.72) | 0.323 | ||||
| TP53 (wild type vs mutant) | 0.97 (0.67-1.4) | 0.860 | 0.91 (0.61-1.35) | 0.630 | ||||
| APC (wild type vs mutant) | 1.08 (0.7-1.66) | 0.719 | 1.27 (0.82-1.97) | 0.276 | ||||
| KRAS (wild type vs mutant) | 1.17 (0.81-1.67) | 0.408 | 0.7 (0.48-1.03) | 0.070 | 0.755 (0.48-1.19) | 0.223 | ||
| NRAS (wild type vs mutant) | 0.86 (0.44-1.7) | 0.663 | 1.55 (0.63-3.81) | 0.339 | ||||
| BRAF (wild type vs mutant) | 0.76 (0.46-1.23) | 0.264 | 1.12 (0.6-2.08) | 0.729 | ||||
| MSI status (MSS vs MSI) | 0.52 (0.29-0.93) | 0.027 | 0.31 (0.13-0.76) | 0.01 | 0.79 (0.39-1.62) | 0.526 | ||
| Vascular invasion (with vs without) | 2.28 (1.52-3.41) | 0.000 | 0.74 (0.31-1.77) | 0.494 | 1.77 (1.14-2.75) | 0.012 | 1.18 (0.64-2.17) | 0.598 |
| Lymphovascular invasion (with vs without) | 2 (1.36-2.95) | 0.000 | 1.86 (0.68-5.11) | 0.228 | 1.66 (1.12-2.47) | 0.012 | 0.78 (0.42-1.44) | 0.420 |
| Perineural invasion (with vs without) | 1.56 (0.8 - 3.03) | 0.188 | 1.61 (0.83-3.12) | 0.157 | ||||
| Lymph node count | ||||||||
| 0-11 | 1 | 1 | 1 | 1 | ||||
| 12-23 | 0.55 (0.34-0.89) | 0.016 | 0.67 (0.10-4.70) | 0.688 | 0.68 (0.38-1.2) | 0.181 | 2.32 (1.13-4.74) | 0.020 |
| > 24 | 0.46 (0.27-0.77) | 0.003 | 0.18 (0.02-1.51) | 0.114 | 0.58 (0.32-1.06) | 0.076 | 1.44 (0.88-2.36) | 0.140 |
| CNV/MUT (with vs without) | 0.66 (0.31-1.43) | 0.295 | 1.02 (0.38-2.78) | 0.964 | ||||
| WTAPCNV | ||||||||
| Deep deletion | 1 | 1 | ||||||
| Shallow deletion | 0.13 (0.02-1) | 0.050 | 1.92 (0.15-24.62) | 0.615 | 1 | |||
| Diploid | 0.08 (0.01-0.62) | 0.015 | 0.43 (0.03-5.85) | 0.528 | 0.7 (0.41-1.2) | 0.189 | ||
| Gain | 0.11 (0.01-0.82) | 0.032 | 0.47 (0.04-6.24) | 0.57 | 0.9 (0.45-1.78) | 0.764 | ||
| Amplification | 0.19 (0.01-3.11) | 0.247 | NA (0-inf) | 0.999 | 1.43 (0.19-10.83) | 0.727 | ||
| Writer loss eraser gain status | ||||||||
| LNGN | 1 | 1 | 1 | 1 | ||||
| LNGP | 0.64 (0.29-1.42) | 0.273 | 0.51 (0.10-2.48) | 0.4 | 1.04 (0.48-2.27) | 0.917 | 1.19 (0.46-3.07) | 0.730 |
| LPGN | 1.43 (0.95-2.13) | 0.083 | 0.59 (0.21-1.67) | 0.316 | 1.54 (0.97-2.43) | 0.066 | 1.58 (0.90-2.76) | 0.110 |
| LPGP | 0.87 (0.48-1.57) | 0.637 | 0.22 (0.04-1.09) | 0.064 | 1.77 (1.03-3.03) | 0.038 | 1.64 (0.85-3.14) | 0.140 |
CNV: copy number variation; LN(P)GN(P): loss negative (positive) gain negative (positive). OS: overall survival; DFS: disease-free survival.